This second edition of Plant Drug Analysis includes nearly new color photographs of superb quality demonstrating chromatograms of all relevant standard drugs. The atlas will be a useful reference for analyzing plant drugs, identifying unknown drugs or monitoring the purity or constituents of a given drug. All drugs presented meet the standard of the official pharmacopoeia and originate from well defined botanical sources. With this guide one can easily use the technique of thin layer chromatography without previous pharmacognostic training. Only commercially available equipment and reagents are needed, the sources as well as all practical details are given.
Colour, chlorophyll and chromatography
thin layer chromatography
Thin-layer chromatography is a chromatography technique that separates pigments, identifying molecules. While it has many applications in a wide variety of industries, it is a particularly important technique used in forensic labs, helping scientists determine if two pieces of text were written by the same ink, which can often be an indicator of fraud. The technique of chromatography was first used in by scientist Mikhail Tsvet to separate the pigments of plants. Later, in the s, new chromatography techniques began to emerge, including thin-layer chromatography TLC which was also developed for use in separating plant pigments. The method of TLC involves separating non-volatile compounds by their rate of movement through the stationary phase a thin layer of adsorbent material, usually silica gel, but sometimes aluminum oxide or cellulose which is coated onto the glass plate where the process takes place. Since its invention, TLC has evolved over the decades for use in numerous applications.
Thin Layer Chromatography TLC is a technique for the analysis of chemical mixtures, widely used in most laboratories. Although it is fairly basic and easy to perform, TLC is at the same time very practical and provides useful information. Thin-Layer Chromatography. The technique is used to determine if a compound is present in a given mixture or to qualitatively assess the purity of a substance. By observing the disappearance of a reagent or the appearance of a product, TLC is often used to monitor chemical reactions.
Chromatography is used to separate mixtures of substances into their components. All forms of chromatography work on the same principle. They all have a stationary phase a solid, or a liquid supported on a solid and a mobile phase a liquid or a gas. The mobile phase flows through the stationary phase and carries the components of the mixture with it.